Diagnostic Values of Salivary miRNA-21 for Early Laryngeal Squamous Cell Carcinoma

Abstract

Objective To explore the diagnostic value of salivary miR-21 for patients with laryngeal squamous cell carcinoma(LSCC). Methods We collected saliva samples from 20 LSCC patients, 15 vocal cord polyp patients and 15 healthy controls were collected. Real-time quantitative reverse transcription PCR was applied to compare the relative expression of miR-21. The changes of miRNA-21 in six LSCC patients before and six months after operation was compared through monitoring analysis. Results The expression of salivary miRNA-21 in LSCC patients was significantly higher than that in healthy control group (P=0.036). The diagnostic value of miRNA-21 for LSCC by ROC curve analysis showed that the area under the curve (AUC) of the miRNA-21 in saliva was 0.792 (95%CI: 0.5353-0.885, P=0.036). The relative expression level of miRNA-21 was significantly decreased in six LSCC patients beforeand six months after operation(P=0.041). Conclusion miRNA-21 is highly expressed in the saliva of patients with laryngeal squamous cell carcinoma, and it could be used as an indicator of early diagnosis of laryngeal squamous cell carcinoma in future.

Keywords

• laryngeal squamous cell carcinoma
• saliva
• mirna-21
• diagnosis
• noninvasive