Effect of different hormonal combination on In vitro direct shoot regeneration of basil (Ocimum basilicum)

Abstract

Basil (Ocimum basilicum ) is an annual, herbaceous plants belonging to the family of lamiaceae. Basil is used to as a herb, a spice as well as fresh vegetables. The present study aimed to investigate the effects of different concentrations of growth regulators such as auxin and cytokinin to identify the best hormonal concentration to obtain the highest yield of in vitro produced plantlets using nodal explants. Experiment was performed in factorial completely randomized design, using the basic MS. In this experiment the effects of different concentrations of BAP(0, 2/5, 5 and 7/5 mg/l) and indol acetic acid (0, 0/1 and 0/5 mg/l) was studied alone or together. Regenetaion percent, number of regeneration, vitrification and rooting evaluated. The results showed that the highest percentage and number of regeneration obtained in the medium included 2/5 mg/l BAP concentration and 0 mg/l IAA, respectively. Vitrification was increased with increasing BAP concentration, maximum rooting was observed in the medium containing 0/5 mg/l IAA.

Keywords

• basil
• nodal
• regeneration
• vitrification
• in vitro