Sub-nanomolar sensitive GZnP3 reveals TRPML1-mediated neuronal Zn2+ signals

Taylor F. Minckley; Chen Zhang; Dylan H. Fudge; Anna M. Dischler; Kate D. LeJeune; Haoxing Xu; Yan Qin

doi:10.1038/s41467-019-12761-x

Nature Communications (Oct 2019)

Sub-nanomolar sensitive GZnP3 reveals TRPML1-mediated neuronal Zn2+ signals

Taylor F. Minckley,
Chen Zhang,
Dylan H. Fudge,
Anna M. Dischler,
Kate D. LeJeune,
Haoxing Xu,
Yan Qin

Affiliations

Taylor F. Minckley: Department of Biological Sciences, University of Denver
Chen Zhang: Department of Biological Sciences, University of Denver
Dylan H. Fudge: Department of Biological Sciences, University of Denver
Anna M. Dischler: Department of Biological Sciences, University of Denver
Kate D. LeJeune: Department of Biological Sciences, University of Denver
Haoxing Xu: Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 4114 Biological Sciences Building, 1105 North University
Yan Qin: Department of Biological Sciences, University of Denver

DOI: https://doi.org/10.1038/s41467-019-12761-x
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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Numerous fluorescent Zn2+ sensors are available but most are unsuitable to detect physiological dynamics of cytosolic Zn2+. In this study, the authors present a genetically encoded sensor with sub-nanomolar sensitivity and show that Zn2 + is released from endolysosomal vesicles via TRPML1 in neurons.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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