Volatiles Profile of the Floral Organs of a New Hybrid <i>Cymbidium</i>, ‘Sunny Bell’ Using Headspace Solid-Phase Microextraction Gas Chromatography-Mass Spectrometry Analysis

Abstract

Cymbidium is one of the most important genera of flowering plants in the Orchidaceae family, and comprises a wide variety of beautiful and colorful species. Among these, only a few species possess floral scents and flavors. In order to increase the availability of a new Cymbidum hybrid, “Sunny Bell”, this study investigated the volatile floral scents. Volatiles of the floral organs of the new Cymbidium hybrid, “Sunny Bell”, at the full-flowering stage were characterized with headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) analysis. A divinylbenzene-carboxen-polydimethylsiloxane (DVB-CAR-PDMS) fiber gave the best extraction for volatile components. Twenty-three components were identified as the main volatiles for the floral organs of the new Cymbidium hybrid, “Sunny Bell” at the full-flowering stage; twelve compounds in the column, sixteen compounds in the labellum, eleven compounds in the sepals, and nine compounds in the petals were identified. Terpenes are the major source of floral scents in this plant. As a result of GC-MS analysis, the most abundant compound was linalool (69−80%) followed by α-pinene (3−27%), 4,8-dimethyl-1,3,7-nonatriene (5−18%), eucalyptol (6−16%), and 2,6-dimethylnonane (2−16%). The main components were identified as monoterpenes in the petals and sepals, and as monoterpenes and aliphatics in the column and labellum. The results of this study provide a basis for breeding Cymbidium cultivars which exhibit desirable floral scents.

Keywords

• <i>Cymbidium</i> hybrid
• floral scents
• linalool
• monoterpenes
• orchids
• volatile compounds