CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for Plasmodium falciparum malaria transmission research

Sylwia D. Boltryk; Armin Passecker; Arne Alder; Eilidh Carrington; Marga van de Vegte-Bolmer; Geert-Jan van Gemert; Alex van der Starre; Hans-Peter Beck; Robert W. Sauerwein; Taco W. A. Kooij; Nicolas M. B. Brancucci; Nicholas I. Proellochs; Tim-Wolf Gilberger; Till S. Voss

doi:10.1038/s41467-021-24954-4

Nature Communications (Aug 2021)

CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for Plasmodium falciparum malaria transmission research

Sylwia D. Boltryk,
Armin Passecker,
Arne Alder,
Eilidh Carrington,
Marga van de Vegte-Bolmer,
Geert-Jan van Gemert,
Alex van der Starre,
Hans-Peter Beck,
Robert W. Sauerwein,
Taco W. A. Kooij,
Nicolas M. B. Brancucci,
Nicholas I. Proellochs,
Tim-Wolf Gilberger,
Till S. Voss

Affiliations

Sylwia D. Boltryk: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute
Armin Passecker: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute
Arne Alder: Centre for Structural Systems Biology
Eilidh Carrington: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute
Marga van de Vegte-Bolmer: Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Geert-Jan van Gemert: Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Alex van der Starre: Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Hans-Peter Beck: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute
Robert W. Sauerwein: Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Taco W. A. Kooij: Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Nicolas M. B. Brancucci: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute
Nicholas I. Proellochs: Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Tim-Wolf Gilberger: Centre for Structural Systems Biology
Till S. Voss: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute

DOI: https://doi.org/10.1038/s41467-021-24954-4
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 16

Abstract

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During each replication cycle of P. falciparum in the human bloodstream, a small proportion of parasites commits to sexual development and differentiates into transmission-relevant gametocytes. Applying CRISPR-based genome editing, Boltryk et al. engineer P. falciparum lines with sexual commitment rates of 75% to promote future studies on gametocyte biology.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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