Frontiers in Immunology (Jul 2022)

elk1/miR-462-731 Feedback Loop Regulates Macrophages Polarization and Phagocytosis in Grass Carp (Ctenopharyngodon idella)

  • Yan He,
  • Yan He,
  • Yan He,
  • Yuting Liu,
  • Yuting Liu,
  • Yuting Liu,
  • Yuyue Yang,
  • Yang Liu,
  • Yang Liu,
  • Yang Liu,
  • Xuewen Jia,
  • Xuewen Jia,
  • Xuewen Jia,
  • Yubang Shen,
  • Yubang Shen,
  • Yubang Shen,
  • Xiaoyan Xu,
  • Xiaoyan Xu,
  • Xiaoyan Xu,
  • Jiale Li,
  • Jiale Li,
  • Jiale Li

DOI
https://doi.org/10.3389/fimmu.2022.946857
Journal volume & issue
Vol. 13

Abstract

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MicroRNA clusters are microRNAs (miRNAs) that are distributed in close proximity on chromosomes. In this study, we report a miRNA cluster identified from grass carp (Ctenopharyngodon idella), miR-462-731, which plays a positive role in host antibacterial immunity. The expression of miR-462-731 was disrupted after infection by Aeromonas hydrophila. Transcription factor ETS transcription factor ELK1 was identified to bind to the promoter of the miR-462-731 cluster and suppress its expression. In addition, miR-731 negatively regulates the expression of elk1, forms an elk1/miR-462-731 double negative feedback loop. In addition, we found that miR-731 directly targets ezrin a (ezra), participates in inducing PI3K/AKT signaling in macrophage, to induce macrophage polarization to the M1 phenotype with stronger phagocytosis. Our results demonstrate a novel elk1/miR-462-731 feedback loop. The data deepen our understanding of the relationship between macrophage polarization and phagocytosis in teleost fish.

Keywords