Antioxidant activity and GC-MS profile of Conyza bonariensis L. leaves extract and fractions

Abstract

The 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing power (FRAP), and semivolatile compounds of Conyza bonariensis L. leave extract and fractions are discussed. A methanolic crude extract was obtained through maceration, and subsequently, n-hexane, chloroform, and ethyl acetate fractions were collected using a solvent-solvent partition. Total phenols, flavonoids, and antioxidant activity assays were performed in an ultraviolet-visible (UV-Vis) spectrophotometer, and the results were expressed as Gallic Acid, Quercetin, and Trolox equivalents respectively. The findings achieved indicate that ethyl acetate fraction showed the highest DPPH radical scavenging capacity (90.69±3.16%) at 500 µg mL-1, and reduced the ferric tripyridyltriazine complex (Fe3+-TPTZ) with values between 19.68 and 2,355.37 mg Trolox equivalent (TE) g-1. It was identified 28 phytoconstituents through Gas chromatography-mass spectrometry (GC-MS). The scavenging activity of ethyl acetate fraction could be correlated mostly to the presence of eugenol, trans-isoeugenol, lucenin-2, methyl salicylate, and syringic acid. This study reveals that the ethyl acetate fraction could be used as a good source of antioxidants for health benefits.

Keywords

• bioactive compounds
• dpph
• flavonoid
• fractionation
• frap
• polyphenol