Designing and interpreting 4D tumour spheroid experiments

Ryan J. Murphy; Alexander P. Browning; Gency Gunasingh; Nikolas K. Haass; Matthew J. Simpson

doi:10.1038/s42003-022-03018-3

Communications Biology (Jan 2022)

Designing and interpreting 4D tumour spheroid experiments

Ryan J. Murphy,
Alexander P. Browning,
Gency Gunasingh,
Nikolas K. Haass,
Matthew J. Simpson

Affiliations

Ryan J. Murphy: Mathematical Sciences, Queensland University of Technology
Alexander P. Browning: Mathematical Sciences, Queensland University of Technology
Gency Gunasingh: The University of Queensland Diamantina Institute, The University of Queensland
Nikolas K. Haass: The University of Queensland Diamantina Institute, The University of Queensland
Matthew J. Simpson: Mathematical Sciences, Queensland University of Technology

DOI: https://doi.org/10.1038/s42003-022-03018-3
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 11

Abstract

Read online

Tumour spheroid experiments with real-time fluorescent ubiquitination-based cell cycle imaging are interpreted using mathematical modelling and statistical identifiability analysis. A framework is provided to compare data obtained across different experimental designs and the experimental design choices that provide reliable biological insight are revealed.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal