Genetics Selection Evolution (Jan 2004)

A first generation bovine BAC-based physical map

  • Cribiu Edmond P,
  • Eggen André,
  • Boichard Didier,
  • Taourit Sead,
  • Gautier Mathieu,
  • Save Jean-Claude,
  • Mahé Marie-Françoise,
  • Roig Anne,
  • Schibler Laurent

DOI
https://doi.org/10.1186/1297-9686-36-1-105
Journal volume & issue
Vol. 36, no. 1
pp. 105 – 122

Abstract

Read online

Abstract A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.

Keywords