Effect of Aegle marmelos on the growth of brushite crystals

Abstract

Abstract Background The urinary stone formation is a very common health problem due to the prolonged medication. Calcium Hydrogen Phosphate Dihydrate (CaHPO4.2H2O, CHPD and Brushite) is one of the major constituent of urinary stone crystals. The formation and deposition of brushite crystals is the central cause of recurrent kidney stone disease among the global population. The present study aims to investigate the effect of Aegle marmelos leaves on the growth of brushite crystals to explicate the inhibitory effect of urinary stones from a different point of view. Methods The CHPD crystals were grown by the Single Diffusion Gel growth technique. In order to inhibit the formation of urinary stones, CHPD crystals are grown in the laboratory in the pure form and by adding the prolonged medicine A. marmelos taken especially by diabetic patients. A comparative study of the pure and A. marmelos leaves added crystals has been carried out using the Growth factor, Powder X-ray diffraction, Microstructural parameters, FTIR and SEM-EDAX. Results Total mass of the grown crystals is found to decrease with increasing concentrations of leaf extract of the A. marmelos. On comparing with undoped, crystalline size of the A. marmelos doped samples is reduced. Disappearance of absorption band and peak shift in the FTIR shows the incorporation of functional groups of A. marmelos. The morphology changes of the treated crystals are assessed in SEM. Conclusions The result shows that herbal extracts prepared from A. marmelos have good inhibitory effect on the growth of the brushite urinary stone crystals considered.

Keywords

• Brushite
• Aegle marmelos
• Gel growth
• X-ray diffraction
• Microstructural parameters