Mìkrobìologìâ ì Bìotehnologìâ (Mar 2018)
STUDY OF ANTIMICROBIAL ACTIVITY OF ALKYL (R-ARYL) OXY DIALKYL AMMONIUM SALTS TOWARDS THE REFERENCE STRAINS OF MICROORGANISMS
Abstract
Aim: evaluation of the antimicrobial properties of alkyl (R-aryl) oxy dialkyl ammonium salts towards the reference strains of microorganisms. Methods. The objects of the study were 52 derivatives of alkyl (R-aryl) oxy dialkyl ammonium, synthesized at the Institute of Organic Chemistry of NAS of Ukraine. Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 10231 were used as the test microorganisms. The determination of the antimicrobial activity of these compounds was conducted in 2 stages: the 1-st – screening by diffusion method in the nutrient medium; the 2nd – determination of the minimum inhibitory and bactericidal concentration (MIC and MBC) by the micro-method of serial dilutions. Results. As a result of the screening, it has been detected that antifungal and antistaphylococcal effect has been present in 45 among 52 studied, 10 compounds caused the appearance of growth inhibition zones with the diameter of 15–19 mm. All studied substances were not effective towards gram-negative bacteria. The MIC and MBC values were determined referring to the results of the screening. 26 investigated alkyl salts (R-aryl) oxy dialkyl ammonium surpassed antimycotic effect of fluconazole, serving as a reference preparation. Also, the compounds showed a pronounced antibacterial effect on the S. aureus reference strain. Kc22, Kc1 and Kc3 compounds were the most effective against these microorganisms, their antimicrobial properties were higher or consistent with ampicillin (MIC – 1.62 ± 0.33 μg/ml, and MBC – 2.60 ± 0.65 μg/ml). 25 compounds of all 45 studied showed antimicrobial effect at the level of reference product, that is, their MIC didn’t exceed 7.81 μg/ml. Conclusions. 10 most effective compounds selected on the basis of obtained results for further investigation of the antimicrobial properties of alkyl (R-aryl) oxy dialkyl ammonium salts on clinical isolates in vitro.
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