Thidiazuron induced plant regeneration in callus culture of triploid banana (Musa sp.) ‘Gros Michel’, AAA group

Abstract

Yellow friable calluses of banana (Musa sp.) ‘Gros Michel’, AAA group were initiated from in vitro shoot buds cultured on Murashige and Skoog (MS) solid medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and coconut water (CW). Small spherical, compact calluses were formed after friable calluses were transferred to half-MS liquid media supplemented with 1.5 mg/l 2, 4-D or 1.5 mg/l 2, 4-D in combination with 5% CW or without 2,4-D and CW. However, no shoots were produced from these media. Embryogenic calluses were induced followed by subculture the spherical, compact calluses to half-MS solid medium in the presence of thidiazuron (TDZ). These embryogenic calluses gave rise to shoots on MS germination medium containing 2.0 mg/l α-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzyladenine (BA). Complete banana plants were recovered on MS maturation medium containing 0.5 mg/l BA and 0.2% activated charcoal. Morphological observation of plantlets did not show any abnormality.

Keywords

• banana
• callus culture
• ‘Gros Michel’ AAA group
• organogenesis
• thidiazuron