Effect of thidiazuron and indole-butyric acid in the in vitro propagation of two varieties of strawberry (Oso Grande and Sweet Charlie) from foliar sections

Abstract

Strawberry farmers indicate that yields are low compared to other countries. This effect is due to the fact that they plant seedlings that come from stolons since the strawberries are propagated asexual. This con-ventional form of reproduction has disadvantages such as: decreased yield, loss of fruit quality. An alter-native to solve this problem is micropropagation. The present research evaluated the in vitro behavior of two varieties of strawberry (Oso Grande and Sweet Charlie) from leaf segments with different degrees of maturation (juvenile and adult) in two periods of introduction. To induce in vitro propagation, the culture media were supplemented with growth regulators, using two concentrations 4.54 and 9.08 μM of thidiazuron with the combination of three concentrations of 0, 0.98 and 2.5 μM of acid Indolebutyric acid. Explants of leaf segments showing juvenile tissues (May) came to form buds. The same did not occur with those that came from adult tissue (June), so it can be noted that those that came from juvenile tissue had a better response in vitro. It was determined that the combination of 9.08 μM thidiazuron and 0.98 μM indolbutyric acid was the most suitable for the in vitro propagation of leaf sections of strawberry leaves in both varieties, since in this medium more formation was obtained Of cell aggregates, for the formation of outbreaks compared to the other treatments.

Keywords

• Tidiazuron
• cell aggregates
• callogenesis
• explants.