Control Region-mtDNA heterogeneity of Kalimantan false gharial (Tomistoma schiegelii) population: a preliminary study

Abstract

The preliminary genetic study on Kalimantan false gharial from the wild was reported. Eleven tail scutes were collected fromeleven individuals that originally consisting of two individuals from Kapuas River, one individual from Sentarum Lake, Jelai River,Mapam River, Perian Lake, and Lamandau River, two individuals from Barito River and three individuals from Mahakam River. PCRamplifying and sequencing 451 nucleotides in average that can be aligned at the same length of control region mitochondrial DNA.Among 11 individuals found eight polymorphic sites that consisted four haplotypes (A, B, C, and D) respectively, which is haplotypeA is dominant. Based on phylogenetic tree that constructed by Tamura-Nei parameter, false gharial population in Kalimantan can bedivided into two population groups; there were Central-Eastern Kalimantan population group and Western Kalimantan populationgroup. Based on the hypothesis of landmasses separating between central-eastern Kalimantan and western Kalimantan that known asSchwaner Mountains, the genetic distance D = 1.53% was expected to be equal to 20 million years.

Keywords

• False Gharial
• Tomistoma schlegelii
• Mitochondrial DNA
• Kalimantan