Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria

Yang Liu; Xinyi Wan; Baojun Wang

doi:10.1038/s41467-019-11479-0

Nature Communications (Aug 2019)

Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria

Yang Liu,
Xinyi Wan,
Baojun Wang

Affiliations

Yang Liu: School of Biological Sciences, University of Edinburgh
Xinyi Wan: School of Biological Sciences, University of Edinburgh
Baojun Wang: School of Biological Sciences, University of Edinburgh

DOI: https://doi.org/10.1038/s41467-019-11479-0
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 16

Abstract

Read online

CRISPR activation strategies in bacteria are limited due to the reliance on σ70 promoters. Here the authors demonstrate eukaryote-like gene activation with high dynamic ranges using σ54- dependent promoters.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal