Genome-wide CRISPR screens identify noncanonical translation factor eIF2A as an enhancer of SARS-CoV-2 programmed −1 ribosomal frameshifting

Abstract

Summary: Many positive-strand RNA viruses, including all known coronaviruses, employ programmed −1 ribosomal frameshifting (−1 PRF) to regulate the translation of polycistronic viral RNAs. However, only a few host factors have been shown to regulate −1 PRF. Through a genome-wide CRISPR-Cas9 knockout screen, we have identified host factors that either suppress or enhance severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) –1 PRF. Among them, eukaryotic translation initiation factor 2A (eIF2A) specifically and directly enhances −1 PRF independent of changes in initiation. Consistent with the crucial role of efficient −1 PRF in transcriptase/replicase expression, loss of eIF2A reduces SARS-CoV-2 replication in cells. Furthermore, transcriptome-wide analysis shows that eIF2A preferentially binds CG-rich RNA motifs, including a region within 18S ribosomal RNA near the contacts between the SARS-CoV-2 frameshift-stimulatory element (FSE) and the ribosome. Thus, our results indicate a role for eIF2A in modulating the translation of specific RNAs independent of its role during initiation.

Keywords

• CP: Microbiology