Evaluation of Dry Ice for Short-Term Storage and Transportation of Frozen Boar Semen
Mengqian He,
Lingwei Sun,
Jiehuan Xu,
Caifeng Wu,
Shushan Zhang,
Jun Gao,
Defu Zhang,
Yeqing Gan,
Yi Bian,
Jinliang Wei,
Weijian Zhang,
Wengang Zhang,
Xuejun Han,
Jianjun Dai
Affiliations
Mengqian He
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Lingwei Sun
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Jiehuan Xu
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Caifeng Wu
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Shushan Zhang
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Jun Gao
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Defu Zhang
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Yeqing Gan
Shanghai Jiading Municipal Centre for Disease Control and Prevention, Shanghai 201899, China
Yi Bian
Shanghai Jiading Municipal Centre for Disease Control and Prevention, Shanghai 201899, China
Jinliang Wei
Shanghai Jiading Municipal Centre for Disease Control and Prevention, Shanghai 201899, China
Weijian Zhang
Shanghai Municipal Centre for Disease Control and Prevention, Shanghai 200051, China
Wengang Zhang
Shanghai Municipal Centre for Disease Control and Prevention, Shanghai 200051, China
Xuejun Han
Shanghai Engineering Research Center of Breeding Pig, Shanghai 201106, China
Jianjun Dai
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (−79 °C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 °C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN (p > 0.05). There were no significant changes in antioxidant properties between storage groups either (p > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.
