Evaluation of Cuscuta reflexa seed essential oil on TPA-induced inflammation in mice

Abstract

Objectives: Cuscuta reflexa is parasitic plant of subtropical and temperate areas, commonly named as amarbel or akasvel in India. Present study is to investigate the In-vivoanti-inflammatory potential of C. reflexa seed essential oil. Materials and methods: In this study the essential oil was hydro-distilled from dried seeds and analyzed for chemical profiling and anti-inflammatory activity. In –vivo activity was performed on mice inducing TPA as disease causing agent. Study of biochemical parameter and oxidative stress was performed by taking homogenate of ear pinna of mice. Results: Chemical analysis is done by GC and GC-MS study, we found β-Bisabolene and Hexahydro farnesyl acetone as the major component as 13.2 and 14.2% respectively along with cis-chrysanthenyl acetate, caryophyllene oxide, carotol, caryophyllene oxide. In-vitro activity of cell viability was assessed using RAW 264.7 macrophages, showed no reduction in viable cell in the processed culture. In-vivo it significantly reduces the inflammation by inhibiting pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α). Conclusions: Cuscuta reflexa seed essential oil was found effective in the management of inflammation by encountering the inflammatory mediators (cytokines).

Keywords

• Cuscuta reflexa
• Convolvulaceae
• Gas chromatography
• Anti-inflammatory activity