THE RESULTS OF STUDYING OF THE MATERIAL FROM THE PATIENTS WITH SUSPECTED SEVERE ACUTE RESPIRATORY SYNDROME IN THE FAR EAST OF RUSSIA

Abstract

During April-July, 2003 39 specimens of clinical material from six patients and three dead men with suspected «atypical pneumonia» who were detected on the territory of Khabarovsk, Primorski and Sakhalin regions were investigated. The inoculation of clinical material to cell culture Vero E6 did not show the cytopathogenic effect for coronavirus SARS. The amplificate with sought for molecular weight was obtained in RT-PCR assay with primers SAR1 and BNI in sputum and blood specimens of one of the patients, but it was not cDNA of coronavirus SARS. As a result of additional studies there were established the following diagnosis: HFRS, bronchopneumonia with Clebsiella etiology (postmortem) and leptospirosis (postmortem). The terminal diagnosis for the rest patients were: bronchopneumonia of inferior lobes (3), maxillary sinusitis, ARVI and toxocariasis.

Keywords

• severe acute respiratory syndrome (sars)
• laboratory diagnostics
• extraction of viral rna
• pcr
• far eastern region