Production of tetra-methylpyrazine using engineered Corynebacterium glutamicum

Thomas Eng; Yusuke Sasaki; Robin A. Herbert; Andrew Lau; Jessica Trinh; Yan Chen; Mona Mirsiaghi; Christopher J. Petzold; Aindrila Mukhopadhyay

Metabolic Engineering Communications (Jun 2020)

Production of tetra-methylpyrazine using engineered Corynebacterium glutamicum

Thomas Eng,
Yusuke Sasaki,
Robin A. Herbert,
Andrew Lau,
Jessica Trinh,
Yan Chen,
Mona Mirsiaghi,
Christopher J. Petzold,
Aindrila Mukhopadhyay

Affiliations

Thomas Eng: Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Yusuke Sasaki: Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; Graduate School of Advanced Integrated Studies in Human Survivability, Kyoto University, Sakyo-ku, Kyoto, Japan; Japan Society for the Promotion of Science, Sakyo-ku, Kyoto, Japan
Robin A. Herbert: Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Andrew Lau: Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Jessica Trinh: Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Yan Chen: Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Mona Mirsiaghi: Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; Advanced Biofuels Process Demonstration Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, USA
Christopher J. Petzold: Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Aindrila Mukhopadhyay: Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; Corresponding author. Joint BioEnergy Institute, Emeryville, CA, USA.

Journal volume & issue: Vol. 10
p. e00115

Abstract

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Corynebacterium glutamicum ATCC 13032 is an established and industrially-relevant microbial host that has been utilized for the expression of many desirable bioproducts. Tetra-methylpyrazine (TMP) is a naturally occurring alkylpyrazine with broad applications spanning fragrances to resins. We identified an engineered strain of C. glutamicum which produces 5 g/L TMP and separately, a strain which can co-produce both TMP and the biofuel compound isopentenol. Ionic liquids also stimulate TMP production in engineered strains. Using a fed batch-mode feeding strategy, ionic liquid stimulated strains produced 2.2 g/L of tetra-methylpyrazine. We show that feedback from a specific heterologous gene pathway on host physiology leads to acetoin accumulation and the production of TMP.

Published in Metabolic Engineering Communications

ISSN: 2214-0301 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Biology (General)
Website: http://www.journals.elsevier.com/metabolic-engineering-communications/

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