Effect of moderate intensity exercise on HDAC4 and CaMKII genes expression in myocardium of male rats

Abstract

Background: Histone Dacetylases-4 (HDAC4) is phosphorylated by calcium-calmodulin-dependent kinase (CaMKII) that plays an important role in cardiac tissue hypertrophy. Therefore, the present study aimed to investigate the effect of moderate intensity exercise on HDAC4 and CaMKII genes expression in myocardial Wistar male rats. Materials and Methods: In this experimental study, 19 adult male rats with 10 weeks of age and weighing of (243±8.2 g) were divided into 2 groups (endurance training=9) and (control=10) based on weight matching. Training program consisted of 6 weeks of endurance training, 5 days per week and moderate intensity. 24 hours after the last training session, cardiac tissue samples were extracted to measure the expression levels of HDAC4 and CaMKII by in vitro Real Time PCR. Data were analyzed using SPSS software version 23 and independent t-test was used. Results: The results showed that the expression of both HDAC4 and CaMKII genes in the exercise group was lower than the control group, significantly (P<0.001). Conclusion: According to the results of the present study, it seems that moderate intensity endurance training can change some factors related to cardiac hypertrophy tissue of rats and can be effective in preventing cardiovascular disease.

Keywords

• moderate intensity endurance training
• hdac4
• camkii
• myocard
• male rats