Nanoscopy of bacterial cells immobilized by holographic optical tweezers

Robin Diekmann; Deanna L. Wolfson; Christoph Spahn; Mike Heilemann; Mark Schüttpelz; Thomas Huser

doi:10.1038/ncomms13711

Nature Communications (Dec 2016)

Nanoscopy of bacterial cells immobilized by holographic optical tweezers

Robin Diekmann,
Deanna L. Wolfson,
Christoph Spahn,
Mike Heilemann,
Mark Schüttpelz,
Thomas Huser

Affiliations

Robin Diekmann: Department of Physics, Biomolecular Photonics, University of Bielefeld
Deanna L. Wolfson: NSF Center for Biophotonics, University of California
Christoph Spahn: Institute of Physical and Theoretical Chemistry, Johann Wolfgang Goethe-University Frankfurt
Mike Heilemann: Institute of Physical and Theoretical Chemistry, Johann Wolfgang Goethe-University Frankfurt
Mark Schüttpelz: Department of Physics, Biomolecular Photonics, University of Bielefeld
Thomas Huser: Department of Physics, Biomolecular Photonics, University of Bielefeld

DOI: https://doi.org/10.1038/ncomms13711
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 7

Abstract

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Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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