Serum metabolomics reveal pathways associated with protective effect of ginsenoside Rg3 on immune stress

Shicheng Bi; Jianjian Shao; Yiwen Qu; Wei Xu; Jun Li; Li Zhang; Wanyu Shi; Liting Cao

Poultry Science (Dec 2022)

Serum metabolomics reveal pathways associated with protective effect of ginsenoside Rg3 on immune stress

Shicheng Bi,
Jianjian Shao,
Yiwen Qu,
Wei Xu,
Jun Li,
Li Zhang,
Wanyu Shi,
Liting Cao

Affiliations

Shicheng Bi: Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. China; Corresponding author:
Jianjian Shao: Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. China
Yiwen Qu: Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. China
Wei Xu: Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, MOA Key Laboratory of Animal Virology, Center for Veterinary Sciences, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China
Jun Li: Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. China
Li Zhang: Immunology Research Center, Medical Research Institute, Southwest University, Rongchang, Chongqing 402460, P. R. China
Wanyu Shi: College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, P. R. China
Liting Cao: Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. China

Journal volume & issue: Vol. 101, no. 12
p. 102187

Abstract

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ABSTRACT: Our previous study has demonstrated that administration of ginsenoside Rg3 ameliorates immune stress by inhibiting inflammatory responses, reducing oxidative damage and upregulating mRNA expression of mTOR, SOD-1, and HO-1. However, the specific mechanism in relation to the protective effect of ginsenoside Rg3 on stressed broilers especially the metabolites alteration remains obscure. The present study aimed to investigate the underlined mechanism in relation to the pathogenesis and protective effect of ginsenoside Rg3 on stressed broilers using liquid chromatograph-mass spectrometry profiling. Eighteen broiler chicks were randomly allocated to 3 treatments: Control, Model and Rg3. Chickens in Rg3 group received intraperitoneally administered 1 mg/kg Rg3 2 h before LPS challenge. Then the broilers were intraperitoneally injection of 250 µg/kg LPS at the age of 12, 14, 33, and 35 d to induce immune stress. Control group was injected with an equivalent amount of sterile saline. At the end of the experiment, the serum was obtained for metabolomics analysis. The changes in serum metabolic profiles were investigated with the application of metabolomics approach. Distinct changes in metabolite patterns in serum were observed by orthogonal partial least square-discriminate analysis. In total, 35 metabolites were identified, among which 17 differential metabolites were found between Control and Model group, and 18 differential metabolites were identified between Model and Rg3 group. Metabolic pathway analysis revealed potential serum metabolites involved in oxidative stress and inflammation, degradation of lipid and protein in broiler chicks with immune stress. In addition, the protective effect of Rg3 on the stressed chicks may be largely mediated by BCAA metabolism, apoptosis and mTOR signaling pathway. These results suggested the potential biomarkers involved in pathogenesis and prevention of stress induced by Escherichia coli lipopolysaccharide.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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