Gas bubbles induced by combined optical and ultrasound energies for high-resolution deep optical microscopy
Jinwoo Kim,
Juhwan Kim,
Haemin Kim,
Jin Ho Chang
Affiliations
Jinwoo Kim
Department of Electronic Engineering and Computer Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Dalseong-gun, Daegu 42988, South Korea
Juhwan Kim
Department of Electronic Engineering and Computer Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Dalseong-gun, Daegu 42988, South Korea
Haemin Kim
Advanced Photonics Research Institute, Gwangju Institute of Science and Technology (GIST), Buk-gu, Gwangju 61005, South Korea
Jin Ho Chang
Department of Electronic Engineering and Computer Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Dalseong-gun, Daegu 42988, South Korea
Ultrasound-induced optical clearing microscopy (US-OCM) addresses limited imaging depth in optical microscopy, caused by light scattering in biological tissues. It uses ultrasound-induced gas bubbles to better image biological samples. However, controlling the bubble location using only ultrasound is challenging. This study introduces a novel method, “optrasound,” combining optical and ultrasound energies for precise bubble control. It presents the ultrasound field and uses a focused laser to trigger bubble formation. Optrasound-induced deep microscopy improves light beam width by 3.39 times at a depth of 350 µm because the gas bubbles reduce light scattering. This technique can precisely localize a bubble cloud while matching the US-OCM performance.
