Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity

Francesco Simone Ruggeri; Jerome Charmet; Tadas Kartanas; Quentin Peter; Sean Chia; Johnny Habchi; Christopher M. Dobson; Michele Vendruscolo; Tuomas P. J. Knowles

doi:10.1038/s41467-018-06345-4

Nature Communications (Sep 2018)

Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity

Francesco Simone Ruggeri,
Jerome Charmet,
Tadas Kartanas,
Quentin Peter,
Sean Chia,
Johnny Habchi,
Christopher M. Dobson,
Michele Vendruscolo,
Tuomas P. J. Knowles

Affiliations

Francesco Simone Ruggeri: Department of Chemistry, University of Cambridge
Jerome Charmet: Department of Chemistry, University of Cambridge
Tadas Kartanas: Department of Chemistry, University of Cambridge
Quentin Peter: Department of Chemistry, University of Cambridge
Sean Chia: Department of Chemistry, University of Cambridge
Johnny Habchi: Department of Chemistry, University of Cambridge
Christopher M. Dobson: Department of Chemistry, University of Cambridge
Michele Vendruscolo: Department of Chemistry, University of Cambridge
Tuomas P. J. Knowles: Department of Chemistry, University of Cambridge

DOI: https://doi.org/10.1038/s41467-018-06345-4
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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Manual sample deposition on a substrate can introduce artifacts in quantitative AFM measurements. Here the authors present a microfluidic spray device for reliable deposition of subpicoliter droplets which dry out in milliseconds after landing on the surface, thereby avoiding protein self-assembly.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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