GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTAN

Abstract

Kazakhstan has one of the highest incidence of multiple drug resistant tuberculosis in the world. The goal is to describe isolated of M. tuberculosis circulating in the Southern regions of Kazakhstan basing on MIRU-VNTR-profile and spectrum of gene mutations responsible for resistance to the first and second line drugs. 58 clinical isolates of M. tuberculosis with extensive drug resistance were collected. Genetic analysis was conducted by sequencing of genes of rpoB, katG, embB and inhA-fabG of operon and genes of gyrA, gyrB and rrs. The replacement of asparaginic acid with cysteine in the 94th codon of gyr4 gene was found out, which has not been described in literature before. Mutations dominated in the 315th codon of katG (n = 53; 91.4%), in the 531th codon of rpoB (n = 45; 77.6%), in position 1 401 of A/G gene rrs (n = 33; 56.9%) and in the 94th codon of gyrA. MIRU-VNTR typing showed that the majority of isolates belonged to W-Beijing family (n = 53, 94.4%). W-Beijing family often manifests resistance which can explain the increase of drug resistant tuberculosis incidence in the Republic.

Keywords

• туберкулез
• диагностика
• лекарственная чувствительность
• лекарственная устойчивость возбудителя