Retrospective Evaluation of Gram-Negative Bacteria and Their Antibiotic Susceptibility Isolated From Urine Cultures in Children

Abstract

Purpose: Urinary tract infections are among the most common infections in childhood. In this study, it was aimed to evaluate the factors of urinary tract infection in our hospital and to determine antimicrobial resistance patterns and empirical treatment options. Materials and Methods: The results of urine cultures that were sent from the Department of Pediatrics between 01.01.2015 and 31.12.2020 to the Microbiology Laboratory were evaluated retrospectively. The identification of the bacterial growth was tested in Vitek MS (Biomeriux, France) and the antimicrobial susceptibility of the isolates was tested in Vitek2 Compakt (Biomeriux, France). Results: A total of 4257 urine culture samples were evaluated. The most frequently isolated bacteria were E. coli 62.93% followed by Klebsiella spp. 19.6%, Pseudomonas spp. 5.37%, Proteus spp. 4.48%. The highest resistance rates were determined against ampicillin in E. coli (78.96%) and for cefuroxime (63.11%) in Klebsiella spp isolates. The lowest resistance rate was determined against imipenem and meropenem in E.coli (0.12%, 0.15%) isolates and, amikacin and nitrofurantoin in Klebsiella spp. isolates (6.10%, 2.46%). Conclusion: In order to provide effective treatment for urinary tract infections and to minimize morbidity and long-term complications, the frequency of the pathogens causing UTIs, local or regional antibiotic resistance should be known and empirical treatment should be arranged accordingly.

Keywords

• antibiotic resistance
• pediatrics
• urinary infection