Fine-Mapping of <i>qECL7.1</i>, a Quantitative Trait Locus Contributing to Epicotyl Length in Adzuki Bean (<i>Vigna angularis</i>)
Modester Kachapila,
Yuki Horiuchi,
Hidetaka Nagasawa,
Noe Michihata,
Toru Yoshida,
Yuta Kato,
Paul C. Bethke,
Kiyoaki Kato,
Masahiko Mori
Affiliations
Modester Kachapila
Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada, Obihiro 080-8555, Hokkaido, Japan
Yuki Horiuchi
Tokachi Agricultural Experiment Station, Agricultural Research Department, Hokkaido Research Organization, Memuro 082-0081, Hokkaido, Japan
Hidetaka Nagasawa
Tokachi Agricultural Experiment Station, Agricultural Research Department, Hokkaido Research Organization, Memuro 082-0081, Hokkaido, Japan
Noe Michihata
Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada, Obihiro 080-8555, Hokkaido, Japan
Toru Yoshida
Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada, Obihiro 080-8555, Hokkaido, Japan
Yuta Kato
Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada, Obihiro 080-8555, Hokkaido, Japan
Paul C. Bethke
Vegetable Crops Research Unit, USDA Agricultural Research Service, Madison, WI 53706, USA
Kiyoaki Kato
Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada, Obihiro 080-8555, Hokkaido, Japan
Masahiko Mori
Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada, Obihiro 080-8555, Hokkaido, Japan
Increasing the epicotyl length (ECL) of adzuki bean cultivars enhances the suitability for mechanical weeding during the vegetative stages and harvesting at pod maturity. To explore the genetic control of ECL, and to identify molecular markers that could facilitate breeding for increased ECL, recombinant inbred lines (RILs) were developed from a cross between Toiku161 (long epicotyls) and Chihayahime (ordinary length epicotyls). In this study, four quantitative trait loci (QTLs) were identified for ECL by QTL-seq analysis, one each on chromosomes 2, 7, 10 and 11. Insertion and deletion (InDel)-based mapping also detected QTLs on chromosomes 7, qECL7.1, and 10, qECL10.1. Substitution mapping using InDel, cleaved amplified polymorphic sequence (CAPS), derived cleaved amplified polymorphic sequence (dCAPS), and single nucleotide polymorphism (SNP) markers narrowed the chromosomal location of qECL7.1 to a 418 kb region flanked by DNA markers TC99_10,211,134 bp and TC102_10,628,880 bp. A total of 35 genes were predicted within the qECL7.1 region. The ECL QTLs and molecular markers identified here will contribute towards marker-assisted selection of desirable long ECL genotypes that allow for increased mechanization and more efficient adzuki bean production.
