Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues

Abstract

Objective To observe hypoxic injuries in 7 cell lines derived from different human tissues to identify a cell model that is sensitive to acute hypoxic injury. Methods Seven cell lines derived from different human tissues, namely CCD 841 CoN, GES-1, HUVEC, HEB, HEK-293, HBE and L02, were exposed to different oxygen concentrations (1% and 5%) for 24 h using a hypoxia workstation. CCK-8 and LDH kits were used to assess the cell proliferation and cell injuries. The cells were then treated with rhodiola (0.625 μmol/L, 2.5 mmol/L) and 3-n-butylphthalide (1, 2 μmol/L) at the start of hypoxic exposure to assess the protective effects of the drugs against hypoxic cell injuries. Results Compared with the cells cultured in normal oxygen concentration (21%), HEB cells exposed to 1% O2 for 24 h showed significantly lowered proliferative activity (P < 0.05). Hypoxic exposure to 5% or 1% O2 for 24 h significantly increased LDH activity in the culture supernatant of HEB, HUVEC, HBE, HEK-293, CCD 841 CoN, GES-1 and L02 cells (P < 0.05 or 0.01). In HEB cells cultured under hypoxic conditions, treatment with rhodiola and 3-n-butylphthalide significantly promoted the cell proliferation and improved the cell viability (P < 0.05), and obviously reversed hypoxia-induced increase in LDH activity (P < 0.05 or P < 0.01). Conclusion Among the 7 cell lines tested in this study, HEB cells are the most sensitive to hypoxia and can serve as an ideal model for studying drug interventions of hypoxic cell injuries.

Keywords

• hypoxia
• tissue cells
• cell proliferation
• lactic dehydrogenase