Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells

Marco Orecchioni; Davide Bedognetti; Leon Newman; Claudia Fuoco; Filomena Spada; Wouter Hendrickx; Francesco M. Marincola; Francesco Sgarrella; Artur Filipe Rodrigues; Cécilia Ménard-Moyon; Gianni Cesareni; Kostas Kostarelos; Alberto Bianco; Lucia G Delogu

doi:10.1038/s41467-017-01015-3

Nature Communications (Oct 2017)

Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells

Marco Orecchioni,
Davide Bedognetti,
Leon Newman,
Claudia Fuoco,
Filomena Spada,
Wouter Hendrickx,
Francesco M. Marincola,
Francesco Sgarrella,
Artur Filipe Rodrigues,
Cécilia Ménard-Moyon,
Gianni Cesareni,
Kostas Kostarelos,
Alberto Bianco,
Lucia G Delogu

Affiliations

Marco Orecchioni: Department of Chemistry and Pharmacy University of Sassari
Davide Bedognetti: Immunology and Therapy Section, Division of Translational Medicine, Sidra Medical and Research Center
Leon Newman: Nanomedicine Laboratory, Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester
Claudia Fuoco: Department of Biology, University of Rome Tor Vergata
Filomena Spada: Department of Biology, University of Rome Tor Vergata
Wouter Hendrickx: Immunology and Therapy Section, Division of Translational Medicine, Sidra Medical and Research Center
Francesco M. Marincola: Office of the Chief Research Officer, Sidra Medical and Research Center
Francesco Sgarrella: Department of Chemistry and Pharmacy University of Sassari
Artur Filipe Rodrigues: Nanomedicine Laboratory, Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester
Cécilia Ménard-Moyon: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d’Immunopathologie et Chimie Thérapeutique
Gianni Cesareni: Department of Biology, University of Rome Tor Vergata
Kostas Kostarelos: Nanomedicine Laboratory, Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester
Alberto Bianco: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d’Immunopathologie et Chimie Thérapeutique
Lucia G Delogu: Department of Chemistry and Pharmacy University of Sassari

DOI: https://doi.org/10.1038/s41467-017-01015-3
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 14

Abstract

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Understanding the interaction of nanomaterials and immune cells at the biomolecular level is of great significance in therapeutic applications. Here, the authors investigated the interaction of graphene oxide nanomaterials and several immune cell subpopulations using single-cell mass cytometry and genome-wide transcriptome analysis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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