Expression of MKK7 and Phospho-c-Jun in Glioma and Their Correlation

ZHI Cheng; LAI Miaoling; LIAO Degui; HAO Zhuofang; WANG Yezhong; WU Liqiang; LIU Sisi; ZENG Shulian; HUANG Ziyan; CHEN Danmin; YUAN Zhongmin

doi:10.3971/j.issn.1000-8578.2019.18.1780

Zhongliu Fangzhi Yanjiu (Sep 2019)

Expression of MKK7 and Phospho-c-Jun in Glioma and Their Correlation

ZHI Cheng,
LAI Miaoling,
LIAO Degui,
HAO Zhuofang,
WANG Yezhong,
WU Liqiang,
LIU Sisi,
ZENG Shulian,
HUANG Ziyan,
CHEN Danmin,
YUAN Zhongmin

Affiliations

ZHI Cheng: Department of Pathology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
LAI Miaoling: Department of Pathology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
LIAO Degui: Department of Pathology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
HAO Zhuofang: Department of Pathology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
WANG Yezhong: Department of Neurosurgery, The Second Affiliated Hospital and Institute of Neurosciences of Guangzhou Medical University, Guangzhou 510260, China
WU Liqiang: Department of Neurosurgery, The Second Affiliated Hospital and Institute of Neurosciences of Guangzhou Medical University, Guangzhou 510260, China
LIU Sisi: Department of Neurosurgery, The Second Affiliated Hospital and Institute of Neurosciences of Guangzhou Medical University, Guangzhou 510260, China
ZENG Shulian: Department of Neurosurgery, The Second Affiliated Hospital and Institute of Neurosciences of Guangzhou Medical University, Guangzhou 510260, China
HUANG Ziyan: Department of Neurosurgery, The Second Affiliated Hospital and Institute of Neurosciences of Guangzhou Medical University, Guangzhou 510260, China
CHEN Danmin: Department of Neurosurgery, The Second Affiliated Hospital and Institute of Neurosciences of Guangzhou Medical University, Guangzhou 510260, China
YUAN Zhongmin: Department of Neurosurgery, The Second Affiliated Hospital and Institute of Neurosciences of Guangzhou Medical University, Guangzhou 510260, China

DOI: https://doi.org/10.3971/j.issn.1000-8578.2019.18.1780
Journal volume & issue: Vol. 46, no. 9
pp. 790 – 795

Abstract

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Objective To investigate the expression of phospho-c-Jun and MKK7 in gliomas and their correlation. Methods We collected and analyzed retrospectively 92 cases of gliomas, including 15 cases of diffuse astrocytoma, 5 cases of oligodendroglioma, 11 cases of anaplastic astrocytoma, 8 cases of anaplastic oligodendroglioma 53 cases of glioblastoma and 25 normal brain tissues adjacent to glioblastoma. The expression of c-Jun, phospho-c-Jun and MKK7 were detected by immunohistochemical staining. Glioma U87 cell line cultured in vitro was transfected with MKK4-siRNA, MKK7-siRNA and control siRNA for 48h, respectively. Western blot was performed to test the expression of c-Jun, phospho-c-Jun and MKK7. Results The expression of p-c-Jun and MKK7 in glioblastoma were significantly higher than those in other glioma and normal brain tissues adjacent to glioblastomas(P=0.000, P=0.000). The expression of MKK7 and phospho-c-Jun were positively correlated with WHO grading of gliomas (r＝0.494, P＝0.000; r=0.606, P=0.000). There was positive correlation between the expression of MKK7 and p-c-Jun (r=0.387, P=0.000). The knockdown of MKK7 suppressed c-Jun activities. Conclusion MKK7 promotes the development of glioblastoma by regulating the activity of c-Jun.

Published in Zhongliu Fangzhi Yanjiu

ISSN: 1000-8578 (Print)
Publisher: Magazine House of Cancer Research on Prevention and Treatment
Country of publisher: China
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: http://www.zlfzyj.com

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