Molecules (Mar 2021)

<i>N</i>-Glycomics of Cerebrospinal Fluid: Method Comparison

  • Byeong Gwan Cho,
  • Cristian D. Gutierrez Reyes,
  • Yehia Mechref

DOI
https://doi.org/10.3390/molecules26061712
Journal volume & issue
Vol. 26, no. 6
p. 1712

Abstract

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Cerebrospinal fluid (CSF) contains valuable biological and neurological information. However, its glycomics analysis is hampered due to the low amount of protein in the biofluid, as has been demonstrated by other glycomics studies using a substantial amount of CSF. In this work, we investigated different N-glycan sample preparation approaches to develop a more sensitive method. These methods, one with an increased amount of buffer solution during the N-glycan release step with a lower amount of sample volume and the other with Filter-Aided N-Glycan Separation (FANGS), were compared with recent work to demonstrate their effectiveness. It was demonstrated that an increased amount of buffer solution showed higher intensity in comparison to the previously published method and FANGS. This suggested that digestion efficiency during the N-glycan release step was not in an optimal condition from the previously published method, and that there is a substantial loss of sample with FANGS when preparing N-glycans from CSF.

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