Two New Coumarins from <em>Micromelum </em><em>f</em><em>alcatum </em>with Cytotoxicity and Brine Shrimp Larvae Toxicity

Abstract

Two new coumarins, 7-methoxy-8-(2-hydroxmethyl-1-O-isovaleryl-4-butenyl)-coumarin (<strong>1</strong>) and 7-methoxy-8-(1-hydroxy-2-O-b-glucopyranosyl-3-methyl-4-butene-1-yl)coumarin (<strong>2</strong>), and twelve known coumarins <strong>3–14</strong> were isolated from the stem bark of <em>Micromelum falcatum</em>. The structures of compounds <strong>1–</strong><strong>14</strong> were elucidated by extensive spectroscopic data analyses. The toxicity of compounds <strong>1–1</strong><strong>4</strong> was tested using a brine shrimp assay and <em>in vitro</em> antiproliferative assay against mammary cancer (F10) and lung cancer (HvEvc) cell lines by the MTT method. Some compounds had moderate activities. All compounds were also tested against the microorganisms <em>Bacillus subtilis</em>, <em>Bacillus thuringiensis</em> and <em>Escherichia coli</em>, but no activity was observed.

Keywords

• &lt;em&gt;Micromelum falcatum&lt;/em&gt;
• coumarins
• brine shrimp larvae toxicity
• cytotoxicity
• antibacterial activity