Targeted volumetric single-molecule localization microscopy of defined presynaptic structures in brain sections

Martin Pauli; Mila M. Paul; Sven Proppert; Achmed Mrestani; Marzieh Sharifi; Felix Repp; Lydia Kürzinger; Philip Kollmannsberger; Markus Sauer; Manfred Heckmann; Anna-Leena Sirén

doi:10.1038/s42003-021-01939-z

Communications Biology (Mar 2021)

Targeted volumetric single-molecule localization microscopy of defined presynaptic structures in brain sections

Martin Pauli,
Mila M. Paul,
Sven Proppert,
Achmed Mrestani,
Marzieh Sharifi,
Felix Repp,
Lydia Kürzinger,
Philip Kollmannsberger,
Markus Sauer,
Manfred Heckmann,
Anna-Leena Sirén

Affiliations

Martin Pauli: Department for Neurophysiology, Institute for Physiology, Julius-Maximilians-University Würzburg
Mila M. Paul: Department for Neurophysiology, Institute for Physiology, Julius-Maximilians-University Würzburg
Sven Proppert: Department for Neurophysiology, Institute for Physiology, Julius-Maximilians-University Würzburg
Achmed Mrestani: Department for Neurophysiology, Institute for Physiology, Julius-Maximilians-University Würzburg
Marzieh Sharifi: Department for Neurophysiology, Institute for Physiology, Julius-Maximilians-University Würzburg
Felix Repp: Department for Neurophysiology, Institute for Physiology, Julius-Maximilians-University Würzburg
Lydia Kürzinger: Department for Neurophysiology, Institute for Physiology, Julius-Maximilians-University Würzburg
Philip Kollmannsberger: Center for Computational and Theoretical Biology, Julius-Maximilians-University Würzburg
Markus Sauer: Department of Biotechnology and Biophysics, Biocenter, Julius-Maximilians-University Würzburg
Manfred Heckmann: Department for Neurophysiology, Institute for Physiology, Julius-Maximilians-University Würzburg
Anna-Leena Sirén: Department of Neurosurgery, University Hospital of Würzburg

DOI: https://doi.org/10.1038/s42003-021-01939-z
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 13

Abstract

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Pauli et al. develop targeted volumetric dSTORM in order to image large hippocampal mossy fiber boutons (MFBs) in brain slices. They can identify synaptic targets of individual MFBs and measured size and density of Bassoon clusters within individual untruncated MFBs at nanoscopic resolution.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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