Frontiers in Immunology (Dec 2016)

NKG2A-Expressing Natural Killer Cells Dominate the Response to Autologous Lymphoblastoid Cells Infected with Epstein-Barr Virus

  • Olivia Hatton,
  • Olivia Hatton,
  • Dara Marie Strauss-Albee,
  • Nancy Q Zhao,
  • Mikel D Haggadone,
  • Judith Shanika Pelpola,
  • Sheri M Krams,
  • Sheri M Krams,
  • Olivia M Martinez,
  • Olivia M Martinez,
  • Catherine A Blish,
  • Catherine A Blish

DOI
https://doi.org/10.3389/fimmu.2016.00607
Journal volume & issue
Vol. 7

Abstract

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Epstein-Barr virus (EBV) is a human -herpesvirus that establishes latency and lifelong infection in host B cells while achieving a balance with the host immune response. When the immune system is perturbed through immunosuppression or immunodeficiency, however, these latently-infected B cells can give rise to aggressive B cell lymphomas. Natural killer (NK) cells are regarded as critical in the early immune response to viral infection, but their role in controlling expansion of infected B cells is not understood. Here we report that NK cells from healthy human donors display increased killing of autologous B lymphoblastoid cell lines (LCL) harboring latent EBV compared to primary B cells. Co-culture of NK cells with autologous EBV+ LCL identifies an NK cell population that produces IFN-gamma and mobilizes the cytotoxic granule protein CD107a. Multi-parameter flow cytometry and Boolean analysis reveals that these functional cells are enriched for expression of the NK cell receptor NKG2A. Further, NKG2A+ NK cells more efficiently lyse autologous LCL than do NKG2A- NK cells. More specifically, NKG2A+2B4+CD16-CD57-NKG2C-NKG2D+ cells constitute the predominant NK cell population that responds to latently-infected autologous EBV+ B cells. Thus, a subset of NK cells is enhanced for the ability to recognize and eliminate autologous, EBV-infected transformed cells, laying the groundwork for harnessing this subset for therapeutic use in EBV+ malignancies.

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