Effect of Hydroalcoholic Extract of Anethum graveolens Seed on Lipopolysaccharide-Induced Liver Damage in the Rat

Abstract

Background and Aim: Oxidative stress and inflammatory response play an important role in the development of lipopolysaccharide (LPS)-induced acute liver dysfunction. The purpose of this study was to investigate the effect of hydroalcoholic extract of dill seed on liver damage induced by LPS. Materials and Methods: In this study, 32 rats weighing between 200 and 250 g were divided into four groups: control, control treated with dill hydroalcoholic extract, rats with liver damage، and rats with liver damage treated with dill hydroalcoholic extract. Dill hydroalcoholic extract was administered at a dose of 200 mg/kg. LPS was used to induce liver damage. Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), superoxide dismutase (SOD), and catalase level of malondialdehyde (MDA) and tumor necrosis factor a (TNF-α) were measured. The differences between groups were analyzed using one-way ANOVA and Tukey's tests. Results: The levels of ALT, AST, ALP, TNF-α and MDA significantly increased and activities of SOD and catalase significantly decreased in the LPS group compared to those in the control group. Treatment with hydroalcoholic extract of dill significantly decreased ALT, AST, ALP, TNF-α and MDA and significantly increased SOD activity compared to those in the LPS group. Conclusion: Hydroalcoholic extract of dill showed a protective effect against liver dysfunction caused by LPS which is due to its anti-inflammatory and antioxidant properties.

Keywords

• lipopolysaccharide
• liver
• anethum graveolens
• oxidative stress
• inflammation