Effectiveness of Direct Laser Interference Patterning and Peptide Immobilization on Endothelial Cell Migration for Cardio-Vascular Applications: An In Vitro Study
Romain Schieber,
Carlos Mas-Moruno,
Federico Lasserre,
Joan Josep Roa,
Maria-Pau Ginebra,
Frank Mücklich,
Marta Pegueroles
Affiliations
Romain Schieber
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Barcelona East School of Engineering (EEBE), Universitat Politècnica de Catalunya (UPC), Av. Eduard Maristany, 10-14, 08019 Barcelona, Spain
Carlos Mas-Moruno
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Barcelona East School of Engineering (EEBE), Universitat Politècnica de Catalunya (UPC), Av. Eduard Maristany, 10-14, 08019 Barcelona, Spain
Federico Lasserre
Chair of Functional Materials, Faculty of Natural Sciences and Technology, Saarland University, 66123 Saarbrücken, Germany
Joan Josep Roa
Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain
Maria-Pau Ginebra
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Barcelona East School of Engineering (EEBE), Universitat Politècnica de Catalunya (UPC), Av. Eduard Maristany, 10-14, 08019 Barcelona, Spain
Frank Mücklich
Chair of Functional Materials, Faculty of Natural Sciences and Technology, Saarland University, 66123 Saarbrücken, Germany
Marta Pegueroles
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Barcelona East School of Engineering (EEBE), Universitat Politècnica de Catalunya (UPC), Av. Eduard Maristany, 10-14, 08019 Barcelona, Spain
Endothelial coverage of an exposed cardiovascular stent surface leads to the occurrence of restenosis and late-stent thrombosis several months after implantation. To overcome this difficulty, modification of stent surfaces with topographical or biochemical features may be performed to increase endothelial cells’ (ECs) adhesion and/or migration. This work combines both strategies on cobalt-chromium (CoCr) alloy and studies the potential synergistic effect of linear patterned surfaces that are obtained by direct laser interference patterning (DLIP), coupled with the use of Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) peptides. An extensive characterization of the modified surfaces was performed by using AFM, XPS, surface charge, electrochemical analysis and fluorescent methods. The biological response was studied in terms of EC adhesion, migration and proliferation assays. CoCr surfaces were successfully patterned with a periodicity of 10 µm and two different depths, D (≈79 and 762 nm). RGD and YIGSR were immobilized on the surfaces by CPTES silanization. Early EC adhesion was increased on the peptide-functionalized surfaces, especially for YIGSR compared to RGD. High-depth patterns generated 80% of ECs’ alignment within the topographical lines and enhanced EC migration. It is noteworthy that the combined use of the two strategies synergistically accelerated the ECs’ migration and proliferation, proving the potential of this strategy to enhance stent endothelialization.
