Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay

Florencia del Puerto; Mónica Ozorio; Beatriz Trinidad; Nidia Martínez; Martha Torales; Luciano Franco; Luis Ferreira; Ninfa Vera de Bilbao

Parasite Epidemiology and Control (Nov 2020)

Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay

Florencia del Puerto,
Mónica Ozorio,
Beatriz Trinidad,
Nidia Martínez,
Martha Torales,
Luciano Franco,
Luis Ferreira,
Ninfa Vera de Bilbao

Affiliations

Florencia del Puerto: National University of Asunción, Research Institute in Health Science (IICS-UNA), Department of Tropical Medicine, San Lorenzo, Paraguay; Corresponding author at: Department of Tropical Medicine, Research Institute in Health Science (IICS-UNA), National University of Asunción, Campus UNA, San Lorenzo, Paraguay.
Mónica Ozorio: National Program of Malaria Control, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay
Beatriz Trinidad: National Program of Malaria Control, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay
Nidia Martínez: Department of Entomology, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay
Martha Torales: Technical Management Office, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay
Luciano Franco: Department of Entomology, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay
Luis Ferreira: Department of Entomology, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay
Ninfa Vera de Bilbao: National University of Asunción, Research Institute in Health Science (IICS-UNA), Department of Tropical Medicine, San Lorenzo, Paraguay

Journal volume & issue: Vol. 11
p. e00174

Abstract

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In Paraguay, no cases of Malaria have been recorded since 2011. Microscopy and the SnM-PCR technique were implemented to detect and characterize Plasmodium spp. both in mosquitoes and in humans residing in historically endemic areas of Paraguay, to evaluate the possibility of finding asymptomatic cases and/or Plasmodium parasites circulating in anophelines. Between 2013 and 2015, 361 human blood samples were collected on filter paper, and between 2016 and 2017, 938 female Anopheles mosquitoes were captured in 15 Paraguayan localities. All the diagnostic techniques showed negative results. We observed no asymptomatic case or Plasmodium circulating in vectors.

Published in Parasite Epidemiology and Control

ISSN: 2405-6731 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: https://www.journals.elsevier.com/parasite-epidemiology-and-control/

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