The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection

Jing Jing Wang; Ying Liu; Zhou Ding; Le Zhang; Caiqin Han; Changchun Yan; Eric Amador; Liqin Yuan; Ying Wu; Chunyuan Song; Ying Liu; Wei Chen

Bioactive Materials (Nov 2022)

The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection

Jing Jing Wang,
Ying Liu,
Zhou Ding,
Le Zhang,
Caiqin Han,
Changchun Yan,
Eric Amador,
Liqin Yuan,
Ying Wu,
Chunyuan Song,
Ying Liu,
Wei Chen

Affiliations

Jing Jing Wang: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China
Ying Liu: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China
Zhou Ding: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China
Le Zhang: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China
Caiqin Han: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China
Changchun Yan: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China
Eric Amador: Department of Physics, The University of Texas at Arlington, Arlington, TX, 76019-0059, USA
Liqin Yuan: Department of General Surgery, The Second Xiangya Hospital, Central South University, ChangSha, Hu'nan, 410011, China
Ying Wu: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China
Chunyuan Song: Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing, 210023, China
Ying Liu: Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China; Corresponding author.
Wei Chen: Department of Physics, The University of Texas at Arlington, Arlington, TX, 76019-0059, USA; Medical Technology Research Centre, Chelmsford Campus, Anglia Ruskin University, Chelmsford, CM1 1SQ, UK; Corresponding author.

Journal volume & issue: Vol. 17
pp. 360 – 368

Abstract

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Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS2 (QD-MB @MoS2) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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