Preclinical animal study of electrospun poly (l-lactide-co-caprolactone) and formulated porcine fibrinogen for full-thickness diabetic wound regeneration

Guili Wang; Shuai Ju; Xiaoyan Li; Yunmin Cai; Yao Li; Wenqiang Li; Siyuan Zhou; Hongbing He; Zhihui Dong; Weiguo Fu

Biomedicine & Pharmacotherapy (Jun 2023)

Preclinical animal study of electrospun poly (l-lactide-co-caprolactone) and formulated porcine fibrinogen for full-thickness diabetic wound regeneration

Guili Wang,
Shuai Ju,
Xiaoyan Li,
Yunmin Cai,
Yao Li,
Wenqiang Li,
Siyuan Zhou,
Hongbing He,
Zhihui Dong,
Weiguo Fu

Affiliations

Guili Wang: Department of Vascular and Wound Center, Jinshan Hospital, Fudan University, Shanghai 200032, China
Shuai Ju: Department of Vascular and Wound Center, Jinshan Hospital, Fudan University, Shanghai 200032, China
Xiaoyan Li: Department of Vascular and Wound Center, Jinshan Hospital, Fudan University, Shanghai 200032, China
Yunmin Cai: Department of Vascular and Wound Center, Jinshan Hospital, Fudan University, Shanghai 200032, China
Yao Li: Department of Vascular and Wound Center, Jinshan Hospital, Fudan University, Shanghai 200032, China
Wenqiang Li: Department of Vascular and Wound Center, Jinshan Hospital, Fudan University, Shanghai 200032, China
Siyuan Zhou: Department of Vascular and Wound Center, Jinshan Hospital, Fudan University, Shanghai 200032, China
Hongbing He: PINE&POWER Biotech Co., Ltd, Shanghai, China; Department of Vascular Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China; Vascular Surgery Institute of Fudan University, Shanghai 200032, China
Zhihui Dong: Department of Vascular and Wound Center, Jinshan Hospital, Fudan University, Shanghai 200032, China; Department of Vascular Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China; Vascular Surgery Institute of Fudan University, Shanghai 200032, China; Correspondence to: 180 Fenglin Road, Xuhui District, Shanghai 200032, China.
Weiguo Fu: Department of Vascular Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China; Vascular Surgery Institute of Fudan University, Shanghai 200032, China

Journal volume & issue: Vol. 162
p. 114734

Abstract

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Diabetic foot ulcer is one of the most serious chronic complications of diabetes mellitus. It may lead to amputation of the lower extremities for diabetics. Our study was to evaluate the effect of electrospun poly (L-lactide-co-caprolactone) and formulated porcine fibrinogen (PLCL/Fg) wound dressing on animal wound model. A blend ratio of PLCL/Fg scaffold was 4 (PLCL):1 (Fg). The scanning electron microscopy findings showed that the fibers’ diameter was 122.5 ± 80.3 nm, and the tensile strength was 9.2 ± 0.2 MPa. In-vivo study of the hog normal model demonstrated that PLCL/Fg dressing had better biocompatibility, degradability, and ability to restore the skin's normal structure. We evaluated the wound healing processes in the rat diabetic model by macroscopic observation and histological observation at 1, 2, and 3 post-operation weeks. In our study, the PLCL/Fg group performed better 3 weeks after surgery, in terms of macroscopic healing and scarring. After surgery, the PLCL/Fg group showed better fibroblast accumulation, tissue granulation, and collagen expression than the control group. Topical treatment with PLCL/Fg dressing effectively enhanced wound healing in both normal and hyperglycemic conditions, suggesting that it may possess wound-healing potential.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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