A sodium alginate - silk fibroin biosponge loaded with thrombin: Effective hemostasis and wound healing
Yansen Li,
Ming Li,
Chang Li,
Jing Chang,
Yuwen Hui,
Chuanlin Wang,
Wei Guo,
Zhulin Li
Affiliations
Yansen Li
Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100069, China
Ming Li
Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China; Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China
Chang Li
Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China; Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China
Jing Chang
Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China; Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China
Yuwen Hui
Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China; Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China
Chuanlin Wang
Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China; Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China; Corresponding author. Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China.
In trauma first aid, rapid hemostasis is a priority, extricating patients from hemorrhagic shock and infection risks. This paper explores novel hemostatic materials, using ion-crosslinking and freeze-drying techniques. Iterative experiments determined optimal conditions for the temperature-variable mixing-freeze-drying chemical reaction of sodium alginate (SA)/silk fibroin (SF). We used SA, SA/SF, SA/SF-TB and commercial hemostatic sponge control samples to perform hemostasis experiments on rat liver injury and femoral artery injury models, and to perform wound healing experiments on rat back full-layer skin. The results showed that the hemostatic time and blood loss of SA/SF-TB group rats (liver hemorrhage model: 397.17 ± 34.80 mg, 77.83 ± 7.41 s; Femoral artery bleeding model: 940.33 ± 41.93 mg, 96.83 ± 4.07 s) was significantly better than other experimental groups, and similar to the commercial group. The wound healing experiment showed that the new granulation tissue thickness of SA/SF-TB group was thicker (380.39 ± 28.56 μm) at day 14. In addition, the material properties and biocompatibility of sponges were characterized by cell experiments and in vivo embedding experiments. All the results showed that the SA/SF-TB hemostatic sponge prepared in this study could not only seal the wound quickly and stop bleeding, but also promote the growth of epidermal cells and fibroblasts and accelerate wound healing. This new material solves the shortcomings of traditional materials such as low stability, limited shelf life, high unit price, and has good biocompatibility, easy preparation, rapid hemostasis and other excellent properties. Therefore, this innovative hemostatic material has great prospects and potential in clinical applications.
