Preliminary detection of alanine aminotransferase before blood donation

Abstract

Objective To explore the optimized strategy of alanine aminotransferase(ALT) screening before blood donation, in order to reduce the waste of blood resources. Methods The blood donation scrapping of one blood center in Chongqing from January to April 2020 was analyzed retrospectively. The correlation between ALT test results by dry chemistry method and rate method in 500 blood donors, with ALT level close to the limit, was analyzed, and the abnormal rates of retest were stratified by initial results of ALT dry chemical method. Results Among blood donors enrolled in this study, the rate of ALT abnormality(0.89%, 43/4 846)was significantly higher than that of HBV (0.25%, 12/4 846), HCV (0.43%, 21/4 846) and HIV (0.19%, 9/4 846)(P<0.05). For the samples with pre-donation ALT close to the limit (40 U/L≤ALT≤50 U/L), a weak correlation between the results of dry chemistry method and rate method was observed, with correlation coefficient at 0.33 (P<0.05). The abnormal rates in retest were significantly higher in 45 U/L≤ ALT ≤50 U/L (10.7%, 22/206) group than that in the ALT < 45U/L group(P<0.05). Conclusion The ALT limit before blood donation should be set to 45 U/L. For blood donors with ALT within the range of (45~50) U / L, blood donation should be deferred until they passed retests by rate method.

Keywords

• blood donor
• alanine aminotransferase
• dry chemical method
• rate method