BIOSYNTHESIS, CHARACTERIZATION AND ANTIPARKINSON ACTIVITY OF MAGNETITE-INDONESIAN VELVET BEANS (Mucuna pruriens L.) NANOPARTICLES

Abstract

The aims of the present study were to synthesize magnetite nanoparticles mediated by the extract of velvet bean (Mucuna pruriens) seed from Indonesia and evaluate its anticataleptic effect on haloperidol-induced catalepsy mice. The magnetite-M. pruriens seed extract nanoparticles (FeMPn) were synthesized by reacting ferric chloride (FeCl3) solution with M. pruriens seed extract. Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX), Transmission Electron Microscopy (TEM), Fourier-Transform Infrared (FTIR) and Thermogravimetric Analysis/Differential Thermal Analysis (TGA/DTA) were used to characterize the FeMPn synthesized. Catalepsy test of FeMPn was performed at dosages of 5, 10, 15, 20 and 25 mg/kg body weight. SEM and TEM images showed the aggregation of FeMPn with a spherical shape and smallest size of 30.5 nm. FTIR spectra confirmed the presence of Fe-O bonding from the absorption band at 557.4 cm-1 . TGA/DTA results confirmed that the addition of Fe leads to increase the thermal stability of the extract. The administration of FeMPn at all dosages was able to lower the catalepsy in mice significantly and it was better than the extract itself with the best dosage was 10 mg/kg body weight. These findings suggest that FeMPn could be a promising option for antiparkinson treatment.

Keywords

• Catalepsy
• Extract
• Magnetite
• Mucuna pruriens
• Nanoparticle.