基于UPLC/Q-TOF MS/MS的芝麻油成分分析Component analysis of sesame oil based on UPLC/Q-TOF MS/MS

Abstract

为发现芝麻油中新成分，通过UPLC/Q-TOF MS/MS对4种芝麻油甲醇提取液进行检测分析。结果表明：4种芝麻油中共发现143种共同物质，其中正离子模式71种，负离子模式72种。共同物质中鉴定出77种物质，其余66种为未鉴定成分；77种鉴定成分中包括脂肪酸及衍生物（8种）、芝麻素、芝麻林素、硬脂酰胺等12种已知成分，其余65种为新发现成分；新发现成分包括油酸酰胺、棕榈酰胺、亚油酰胺、芥酸酰胺、8-甲基-N-香草基壬酰胺、辣椒酰胺、N-硬脂酰丙氨酸等17种脂肪酸酰胺，十六碳烯酸、十八碳烯酸等22种脂肪酸及衍生物，5-脱氧基维醇、天孢菌素等5种类黄酮，马泰树脂醇、银杏酸等21种其他成分。芝麻油中含有大量新成分和未知成分，富含的脂肪酸、脂肪酸酰胺、类黄酮、木脂素等物质可解释其独特生理功能。 In order to discover new components in sesame oil, the methanol extracts of four kinds of sesame oils were detected and analyzed by UPLC/Q-TOF MS/MS. The results showed that 143 common components in sesame oil were found, including 71 in the positive ion mode and 72 in the negative ion mode. A total of 77 components among 143 were identified, and the remaining 66 components were unidentified. The 77 identified components included 12 known components such as sesamin, sesamolin, eight fatty acids and derivatives and stearamide, and 65 newly discovered components. The newly discovered components included 17 fatty amides (erucamide, 8-methyl-N-vanillyl-nonamide, oleamide, palmitamide, linoleamide, capsiamide, N-stearoyl alanine, etc), 22 fatty acids and derivatives (hexadecenoic acid, octadeceneic acid, etc), 5 flavonoids such as 5-deoxyvitanol and tinosporinone, 21 other ingredients such as matairesinol, ginkgoic acid,etc. The sesame oil contains a large number of new and unknown components, and rich in fatty acids, fatty acid amides, flavonoids, lignans and other substances, which can explain its unique physiological functions.

Keywords

• 芝麻油；成分分析；脂肪酸；芝麻素；芝麻林素；硬脂酰胺；油酸酰胺；棕榈酰胺；芥酸酰胺
• sesame oil; component analysis; fatty acid; sesamin; sesamolin; stearamide; oleamide; palmitamide; erucamide