Genome-wide CRISPR/Cas9 deletion screen defines mitochondrial gene essentiality and identifies routes for tumour cell viability in hypoxia

Luke W. Thomas; Cinzia Esposito; Rachel E. Morgan; Stacey Price; Jamie Young; Steven P. Williams; Lucas A. Maddalena; Ultan McDermott; Margaret Ashcroft

doi:10.1038/s42003-021-02098-x

Communications Biology (May 2021)

Genome-wide CRISPR/Cas9 deletion screen defines mitochondrial gene essentiality and identifies routes for tumour cell viability in hypoxia

Luke W. Thomas,
Cinzia Esposito,
Rachel E. Morgan,
Stacey Price,
Jamie Young,
Steven P. Williams,
Lucas A. Maddalena,
Ultan McDermott,
Margaret Ashcroft

Affiliations

Luke W. Thomas: Department of Medicine, University of Cambridge, Cambridge Biomedical Campus
Cinzia Esposito: Department of Medicine, University of Cambridge, Cambridge Biomedical Campus
Rachel E. Morgan: Department of Medicine, University of Cambridge, Cambridge Biomedical Campus
Stacey Price: Wellcome Sanger Institute
Jamie Young: Wellcome Sanger Institute
Steven P. Williams: Wellcome Sanger Institute
Lucas A. Maddalena: Department of Medicine, University of Cambridge, Cambridge Biomedical Campus
Ultan McDermott: Wellcome Sanger Institute
Margaret Ashcroft: Department of Medicine, University of Cambridge, Cambridge Biomedical Campus

DOI: https://doi.org/10.1038/s42003-021-02098-x
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 12

Abstract

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Thomas, Ashcroft and colleagues use genome-wide CRISPR/Cas9 screening under tumour-relevant metabolic conditions to identify genes that are essential in cancer. Mitochondrial gene essentiality is highlighted, and routes for regulating tumour cell viability in hypoxia are identified.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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