A CHEMICAL STUDY FOR THE DETECTION OF PROTEIN AND CARBIDIC MATERIALS AND THE FUNCTIONAL ROLE OF BARRIER Khawia lutei (Cestoda : Lytocestodae ; Caryophyllidea)

Abstract

The study involved the investigation of protein and carbohydrate substances in adult tapeworm Khawia luteiu using histochemical techniques (Ninhydrin- Sheff,Millons reagent and Toluidine-blue). Protein and carbohydrates were found in abundance in reproductive organs ,mesenchyma tissue, vitelline glands and eggs, using Ninhydrin- Sheff and Toluidine blue stains.Also, the muscular system, mainly the longitudinal muscles, showed strong positive reaction with Ninhydrin- Sheff stain and negative reaction with Toluidine blue and Millons reagent. Positive and weakly positive reactions in different tissues and organs were noticed with Millons reagent.The study also showed that the histochemical reactions in the worm varied in strength because the metabolic and physiological activities, such as protection, energy storing, sensitive pulses reception to activate reproductive processes and host immune response resistance.

Keywords

• protein
• carbidic materials
• muscular system