Monitoring the Expression of Leptin Receptor in Avian Model

Abstract

In this study we carried the expression of leptin receptor in organs and abdominal fat of broiler chickens. The leptin receptor is a member of the class I cytokine receptor family, also known as the gpl30 receptor family, although unlike many other family members, the leptin receptor does not form oligomers with gpl30. Activation of leptin receptors directly or indirectly activates multiple signaling pathways that involve kinase-induced phosphorylation of proteins, including JAK2/STAT3, erbB2, ERK, IRS1 and rho/rac. Signaling requires the presence of intact intracellular domains of the receptor. The leptin receptor is an external tyrosine kinase receptor; upon ligand binding each receptor can bind and activate the tyrosine kinase JAK2, which then cross-phosphorylates tyrosine residues in the other receptor in the dimer. In this study we examined the expression of leptin receptor in organs and abdominal fat of broiler chickens. The aim of our experiment was to optimize the methodology for monitoring the expression of the leptin receptor in avian model. We studied the activity of leptin receptor in the liver, heart, spleen and abdominal fat in the final fattening type broiler chickens Ross 308. In heart tissue, spleen, liver at a relatively high concentration of total cDNA in the sample length leptin receptor fragment located in the expected quantities.

Keywords

• broiler chickens
• gene expression
• leptin receptor
• primer pair