iScience (Dec 2023)
CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment
- Kuiying Ma,
- Xuan Wang,
- Linjie Wu,
- Lingling Yu,
- Jinhui Ye,
- Xueling Li,
- Lili Geng,
- Zhongyu Shi,
- Huihui Yang,
- Xijuan Zhang,
- Yongjian Zhang,
- Shuchang Wu,
- Pengfei Yuan,
- Yingchi Zhang,
- Fang Dong,
- Sha Hao,
- Linping Hu,
- Wensheng Wei,
- Riguo Fang,
- Tao Cheng
Affiliations
- Kuiying Ma
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Xuan Wang
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China
- Linjie Wu
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China
- Lingling Yu
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Jinhui Ye
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China
- Xueling Li
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China
- Lili Geng
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Zhongyu Shi
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Huihui Yang
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Xijuan Zhang
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Yongjian Zhang
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Shuchang Wu
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Pengfei Yuan
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China
- Yingchi Zhang
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China
- Fang Dong
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China
- Sha Hao
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China
- Linping Hu
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Tianjin Institutes of Health Science, Tianjin 301600, China; Corresponding author
- Wensheng Wei
- Biomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China; Corresponding author
- Riguo Fang
- EdiGene Inc., Life Science Park, Changping District, Beijing 102206, China; Corresponding author
- Tao Cheng
- State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China; Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, Tianjin 300000, China; Department of Stem Cell & Regenerative Medicine, Peking Union Medical College, Tianjin 300000, China; Tianjin Institutes of Health Science, Tianjin 301600, China; Corresponding author
- Journal volume & issue
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Vol. 26,
no. 12
p. 108561
Abstract
Summary: Hematopoietic stem cell (HSC) surface markers improve the understanding of cell identity and function. Here, we report that human HSCs can be distinguished by their expression of the CEA Cell Adhesion Molecule 5 (CEACAM5, CD66e), which serves as a marker and a regulator of HSC function. CD66e+ cells exhibited a 5.5-fold enrichment for functional long term HSCs compared to CD66e− cells. CD66e+CD34+CD90+CD45RA− cells displayed robust multi-lineage repopulation and serial reconstitution ability in immunodeficient mice compared to CD66e−CD34+CD90+CD45RA−cells. CD66e expression also identified almost all repopulating HSCs within the CD34+CD90+CD45RA− population. Together, these results indicated that CEACAM5 is a marker that enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment.
