Generation of 3 clones of induced pluripotent stem cells (iPSCs) from a patient affected by Crohn's disease

Gaetana Lanzi; Stefania Masneri; Rosalba Monica Ferraro; Elena Genova; Giovanna Piovani; Chiara Barisani; Marco Pelin; Gabriele Stocco; Giuliana Decorti; Matteo Bramuzzo; Silvia Giliani

Stem Cell Research (Oct 2019)

Generation of 3 clones of induced pluripotent stem cells (iPSCs) from a patient affected by Crohn's disease

Gaetana Lanzi,
Stefania Masneri,
Rosalba Monica Ferraro,
Elena Genova,
Giovanna Piovani,
Chiara Barisani,
Marco Pelin,
Gabriele Stocco,
Giuliana Decorti,
Matteo Bramuzzo,
Silvia Giliani

Affiliations

Gaetana Lanzi: A. Nocivelli Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy; Corresponding author.
Stefania Masneri: A. Nocivelli Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Rosalba Monica Ferraro: A. Nocivelli Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Elena Genova: Department of Life Sciences, University of Trieste, 34127 Trieste, Italy
Giovanna Piovani: A. Nocivelli Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Chiara Barisani: A. Nocivelli Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Marco Pelin: Department of Life Sciences, University of Trieste, 34127 Trieste, Italy
Gabriele Stocco: Department of Life Sciences, University of Trieste, 34127 Trieste, Italy
Giuliana Decorti: Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34128 Trieste, Italy; Department of Medical, Surgical and Health Sciences, University of Trieste, 34149 Trieste, Italy
Matteo Bramuzzo: Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34128 Trieste, Italy
Silvia Giliani: A. Nocivelli Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy

Journal volume & issue: Vol. 40

Abstract

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Crohn's disease is a debilitating and incurable chronic inflammatory bowel disease, affecting millions of individuals worldwide, with an increasing frequency. Surgery must be applicable in half of the cases often with a disabling course, and pharmacological treatments may have adverse complications. We generated three isogenic clones of iPSCs from peripheral blood mononuclear cells (PBMCs) of a patient with Crohn's Disease under pharmacological treatment without adverse effects. Sendai virus based vector was used and the iPSCs were characterized for genetic uniqueness, genomic integrity, pluripotency, and differentiation ability. These iPSCs will be a powerful tool to develop tailored therapies.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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