Frontiers in Microbiology (Aug 2020)
Characterization of a Novel Chromosomal Class C β-Lactamase, YOC-1, and Comparative Genomics Analysis of a Multidrug Resistance Plasmid in Yokenella regensburgei W13
- Danying Zhou,
- Danying Zhou,
- Danying Zhou,
- Zhewei Sun,
- Zhewei Sun,
- Junwan Lu,
- Junwan Lu,
- Hongmao Liu,
- Hongmao Liu,
- Wei Lu,
- Wei Lu,
- Hailong Lin,
- Hailong Lin,
- Hailong Lin,
- Xueya Zhang,
- Xueya Zhang,
- Xueya Zhang,
- Qiaoling Li,
- Qiaoling Li,
- Qiaoling Li,
- Wangxiao Zhou,
- Wangxiao Zhou,
- Xinyi Zhu,
- Xinyi Zhu,
- Haili Xu,
- Xi Lin,
- Xi Lin,
- Hailin Zhang,
- Hailin Zhang,
- Teng Xu,
- Kewei Li,
- Kewei Li,
- Qiyu Bao,
- Qiyu Bao,
- Qiyu Bao
Affiliations
- Danying Zhou
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Danying Zhou
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Danying Zhou
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Zhewei Sun
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Zhewei Sun
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Junwan Lu
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Junwan Lu
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Hongmao Liu
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Hongmao Liu
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Wei Lu
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Wei Lu
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Hailong Lin
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Hailong Lin
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Hailong Lin
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Xueya Zhang
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Xueya Zhang
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Xueya Zhang
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Qiaoling Li
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Qiaoling Li
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Qiaoling Li
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Wangxiao Zhou
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Wangxiao Zhou
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Xinyi Zhu
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Xinyi Zhu
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Haili Xu
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Xi Lin
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Xi Lin
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Hailin Zhang
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Hailin Zhang
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Teng Xu
- Institute of Translational Medicine, Baotou Central Hospital, Baotou, China
- Kewei Li
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Kewei Li
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Qiyu Bao
- The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China
- Qiyu Bao
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Qiyu Bao
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2020.02021
- Journal volume & issue
-
Vol. 11
Abstract
Yokenella regensburgei, a member of the family Enterobacteriaceae, is usually isolated from environmental samples and generally resistant to early generations of cephalosporins. To characterize the resistance mechanism of Y. regensburgei strain W13 isolated from the sewage of an animal farm, whole genome sequencing, comparative genomics analysis and molecular cloning were performed. The results showed that a novel chromosomally encoded class C β-lactamase gene with the ability to confer resistance to β-lactam antibiotics, designated blaYOC–1, was identified in the genome of Y. regensburgei W13. Kinetic analysis revealed that the β-lactamase YOC-1 has a broad spectrum of substrates, including penicillins, cefazolin, cefoxitin and cefotaxime. The two functionally characterized β-lactamases with the highest amino acid identities to YOC-1 were CDA-1 (71.69%) and CMY-2 (70.65%). The genetic context of the blaYOC–1-ampR-encoding region was unique compared with the sequences in the NCBI nucleotide database. The plasmid pRYW13-125 of Y. regensburgei W13 harbored 11 resistance genes (blaOXA–10, blaLAP–2, dfrA14, tetA, tetR, cmlA5, floR, sul2, ant(3″)-IIa, arr-2 and qnrS1) within an ∼34 kb multidrug resistance region; these genes were all related to mobile genetic elements. The multidrug resistance region of pYRW13-125 shared the highest identities with those of two plasmids from clinical Klebsiella pneumoniae isolates, indicating the possibility of horizontal transfer of these resistance genes between bacteria of various origins.
Keywords
